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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
05/11/2008 |
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Data da última atualização: |
11/01/2023 |
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Autoria: |
RHEINHEIMER, D. dos S.; GONÇALVES, C. S.; PELLEGRINI, J. B. R. |
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Título: |
Impacto das atividades agropecuárias na qualidade da água. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Ciência & Ambiente, Santa Maria, v. 14, n. 27, p. 85-96, jul./dez., 2003. |
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Idioma: |
Português |
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Conteúdo: |
Conversão de ambientes naturais equilibrados em áreas agricultáveis; Agricultura atual e seus reflexos na qualidade da água; Estudo de caso de contaminação de águas superficiais de uma microbacia fumageira; Manejo dos recursos naturais com possibilidade de melhoria na qualidade da água; Considerações finais. |
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Palavras-Chave: |
Brasil. |
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Thesagro: |
Agricultura Orgânica; Agricultura Sustentável; Água; Contaminação; Qualidade. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00938naa a2200217 a 4500
001 1161095
005 2023-01-11
008 2003 bl uuuu u00u1 u #d
100 1 $aRHEINHEIMER, D. dos S.
245 $aImpacto das atividades agropecuárias na qualidade da água.
260 $c2003
520 $aConversão de ambientes naturais equilibrados em áreas agricultáveis; Agricultura atual e seus reflexos na qualidade da água; Estudo de caso de contaminação de águas superficiais de uma microbacia fumageira; Manejo dos recursos naturais com possibilidade de melhoria na qualidade da água; Considerações finais.
650 $aAgricultura Orgânica
650 $aAgricultura Sustentável
650 $aÁgua
650 $aContaminação
650 $aQualidade
653 $aBrasil
700 1 $aGONÇALVES, C. S.
700 1 $aPELLEGRINI, J. B. R.
773 $tCiência & Ambiente, Santa Maria$gv. 14, n. 27, p. 85-96, jul./dez., 2003.
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Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/11/2022 |
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Data da última atualização: |
16/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICIURA, S. C. M.; OKINO, C. H.; NUCCI, A. DA S.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CINTIA HIROMI OKINO, CPPSE; ALESSANDRA DA SILVA NUCCI, Centro Universitário Central Paulista; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Polymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Parasitology Research, v. 121, p. 3581-3588, 2022. |
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DOI: |
https://doi.org/10.1007/s00436-022-07682-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Chemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus. MenosChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PC... Mostrar Tudo |
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Palavras-Chave: |
Amino acetonitrile derivatives; Anthelmintic resistance; PCR. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus NAL: |
Genotyping. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02402naa a2200265 a 4500
001 2148276
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00436-022-07682-6$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPolymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus.$h[electronic resource]
260 $c2022
520 $aChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus.
650 $aGenotyping
650 $aHaemonchus Contortus
653 $aAmino acetonitrile derivatives
653 $aAnthelmintic resistance
653 $aPCR
700 1 $aOKINO, C. H.
700 1 $aNUCCI, A. DA S.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tParasitology Research$gv. 121, p. 3581-3588, 2022.
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